ELISA

Dynamic Devices has developed a high capacity and high speed enzyme-linked immunosorbent assays (ELISAs) platform from its history with motion control and liquid handling.  This flexible and scalable ELISA workstation is configured with the following:

LabAuto 2010 ELISA System Display:
Configuration:
  1. Oasis LM 1200 with 96 Head and Advanced Gripper
  2. BioTek Washer
  3. BioTek Reader
  4. D2 Reagent Dispenser
  5. D2 Incubator
  6. Oasis Deck Extension

The ELISA system configuration can be slightly modified to accomplish the following assays requiring incubation, washing or reading:

  • Enzyme-linked immunosorbent assays (ELISAs)
  • Enzyme immunoassays (EIAs)
  • Radio immunoassays (RIA) without on-line radioactive detection
  • Fluorescence immunoassays (FIA)
Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays by using antibodies or antigens coupled to an easily-assayed enzyme.  ELISAs can provide a useful measurement of antigen or antibody concentration.  There are two main variations on this method: The ELISA can be used to detect the presence of antigens that are recognized by an antibody or it can be used to test for antibodies that recognize an antigen.
An ELISA is a five-step procedure
  1. coat the microtiter plate wells with antigen
  2. block all unbound sites to prevent false positive results
  3. add antibody to the wells
  4. add anti-mouse IgG conjugated to an enzyme
  5. reaction of a substrate with the enzyme to produce a colored product, thus indicating a positive reaction
There are many different types of ELISAs. One of the most common types of ELISA is "sandwich ELISA."